Western blotting analysis using HLAF antibody (Cat#6179). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLAF antibody (Cat#6179, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
HLA-F; Major Histocompatibility Complex, Class I, F; HLA Class I Histocompatibility Antigen, Alpha Chain F; MHC Class I Antigen F; Leukocyte Antigen F; HLA F Antigen; HLA-5.4; CDA12; HLAF; Non-Classical MHC Class I Antigen; Nonclassical MHC Class I Antigen; HLA-CDA12
Supplied in PBS (pH 7.4) containing 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using HLAF antibody (Cat#6179). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLAF antibody (Cat#6179, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using HLAF antibody (Cat#6179). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLAF antibody (Cat#6179, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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