Western blotting analysis using HLAF antibody (Cat#6179). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLAF antibody (Cat#6179, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
HLA-F; Major Histocompatibility Complex, Class I, F; HLA Class I Histocompatibility Antigen, Alpha Chain F; MHC Class I Antigen F; Leukocyte Antigen F; HLA F Antigen; HLA-5.4; CDA12; HLAF; Non-Classical MHC Class I Antigen; Nonclassical MHC Class I Antigen; HLA-CDA12
Supplied in PBS (pH 7.4) containing 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using HLAF antibody (Cat#6179). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLAF antibody (Cat#6179, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using HLAF antibody (Cat#6179). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLAF antibody (Cat#6179, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000757s ]
SELECT * FROM `goods` WHERE `code` = '6179' LIMIT 1 [ RunTime:0.000515s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000402s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000318s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000373s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 9964 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000301s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000471s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 9964 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000285s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000356s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000266s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.054464s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4466,6196,10251,7474,4763,3696,10401,4101,62,7306) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000895s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000487s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000235s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000520s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000256s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000395s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000423s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000286s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000204s ]
