Western blotting analysis using SUCLG1 antibody (Cat#67034, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using SUCLG1 antibody (Cat#67034, 1:1,000). SUCLG1 expression in wild-type (WT) and SUCLG1 shRNA knockdown (KD) HeLa cells of total cell lysates. β-Tubulin serves as a loading control.
Western blotting analysis using SUCLG1 antibody (Cat#67034). Total lysates (35 μg) from various mouse tissues were loaded and separated by SDS-PAGE. The blot was incubated with SUCLG1 antibody (Cat#67034, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively. Image was developed using PiQ™ ECL Substrate Kit (Cat#636).
Western blotting analysis using SUCLG1 antibody (Cat#67034). Total cell lysates (40μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SUCLG1 antibody (Cat#67034, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:5,000) respectively. Image was developed using PiQ™ ECL Substrate Kit (Cat#636).
Immunocytochemical staining of HeLa cells using SUCLG1 antibody (Cat#67034, 1:50). Top panel: wild type (WT); Bottom panel: SUCLG1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; actin was stained green with Alexa Fluor 488; SUCLG1 was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
