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Home>Product>Primary Antibody

SMAD2 Mouse Oligoclonal Antibody

#6763

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Western blotting analysis using SMAD2 antibody (Cat#6763). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMAD2 antibody (Cat#6763, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with SMAD2 antibody (Cat#6763, 1:1,000). Nuclei were stained blue with DAPI; SMAD2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SMAD2 expression in HepG2 cells using SMAD2 antibody (Cat#6763, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SMAD2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SMAD2
  • Catalog：
    6763
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name SMAD2 Mouse Oligoclonal Antibody
Aliases SMAD2; SMAD Family Member 2; JV18-1; MADR2; MADH2; Mothers Against Decapentaplegic Homolog 2; MAD Homolog 2; HMAD-2; HSMAD2; MAD, Mothers Against Decapentaplegic Homolog 2 (Drosophila); SMAD, Mothers Against DPP Homolog 2 (Drosophila); SMAD, Mothers Against DPP Homolog 2; Sma- And Mad-Related Protein 2; Mothers Against DPP Homolog 2; Mother Against DPP Homolog 2; Mad-Related Protein 2; SMAD 2; CHTD8; Smad2; JV18; LDS6
Background

Gene Name: SMAD2

NCBI Gene Entry: 4087

UniProt Entry: Q15796

Application Information

Molecular Weight: Predicted, 52 kDa; observed, 55 kDa

Clonality: Mouse oligoclonal antibody

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human SMAD2
Isotype Mouse IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using SMAD2 antibody (Cat#6763). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMAD2 antibody (Cat#6763, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with SMAD2 antibody (Cat#6763, 1:1,000). Nuclei were stained blue with DAPI; SMAD2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of SMAD2 expression in HepG2 cells using SMAD2 antibody (Cat#6763, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SMAD2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SMAD2
  • Catalog：
    6763
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using SMAD2 antibody (Cat#6763). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMAD2 antibody (Cat#6763, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with SMAD2 antibody (Cat#6763, 1:1,000). Nuclei were stained blue with DAPI; SMAD2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SMAD2 expression in HepG2 cells using SMAD2 antibody (Cat#6763, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SMAD2.
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