Western blotting analysis using RPRD1B antibody (Cat#6794). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RPRD1B antibody (Cat#6794, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with RPRD1B antibody (Cat#6794, 1:1,000). Nuclei were stained blue with DAPI; RPRD1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
RPRD1B; Regulation Of Nuclear Pre-MRNA Domain Containing 1B; CREPT; DJ1057B20.2; Kub5-Hera; C20orf77; NET60; K-H; Cell-Cycle Related And Expression-Elevated Protein In Tumor; Regulation Of Nuclear Pre-MRNA Domain-Containing Protein 1B; Ku70-Binding Protein 5-Hera; DKFZp434P0735; FLJ44520; Cell Cycle-Related And Expression-Elevated Protein In Tumor; Chromosome 20 Open Reading Frame 77
Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen
Recombinant protein of human RPRD1B
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000 Immunocytochemistry (ICC): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using RPRD1B antibody (Cat#6794). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RPRD1B antibody (Cat#6794, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with RPRD1B antibody (Cat#6794, 1:1,000). Nuclei were stained blue with DAPI; RPRD1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using RPRD1B antibody (Cat#6794). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RPRD1B antibody (Cat#6794, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with RPRD1B antibody (Cat#6794, 1:1,000). Nuclei were stained blue with DAPI; RPRD1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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