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Home>Product>Primary Antibody

YBX1 Recombinant Rabbit mAb

#6870

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Recombinant Antibody
Western blotting analysis using YBX1 antibody (Cat#6870). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with YBX1 antibody (Cat#6870, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with YBX1 antibody (Cat#6870, 1:1,000). Nuclei were stained blue with DAPI; YBX1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    YBX1
  • Catalog：
    6870
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name YBX1 Recombinant Rabbit mAb
Aliases YBX1; Y-Box Binding Protein 1; YB-1; DBPB; YB1; MDR-NF1; NSEP-1; CSDA2; NSEP1; BP-8; CSDB; CCAAT-Binding Transcription Factor I Subunit A; Nuclease-Sensitive Element-Binding Protein 1; Enhancer Factor I Subunit A; Y-Box Transcription Factor; Y-Box-Binding Protein 1; DNA-Binding Protein B; CBF-A; EFI-A; Major Histocompatibility Complex, Class II, Y Box-Binding Protein I; Nuclease Sensitive Element Binding Protein 1
Background

Gene Name: YBX1

NCBI Gene Entry: 4904

UniProt Entry: P67809

Application Information

Molecular Weight: Predicted, 36 kDa; observed, 48 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB1100

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human YBX1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using YBX1 antibody (Cat#6870). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with YBX1 antibody (Cat#6870, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with YBX1 antibody (Cat#6870, 1:1,000). Nuclei were stained blue with DAPI; YBX1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    YBX1
  • Catalog：
    6870
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using YBX1 antibody (Cat#6870). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with YBX1 antibody (Cat#6870, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with YBX1 antibody (Cat#6870, 1:1,000). Nuclei were stained blue with DAPI; YBX1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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