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MT-CO2 Recombinant Rabbit mAb

#6875

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Recombinant Antibody
Western blotting analysis using MT-CO2 antibody (Cat#6875). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MT-CO2 antibody (Cat#6875, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with MT-CO2 antibody (Cat#6875, 1:1,000). Nuclei were stained blue with DAPI; MT-CO2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    MT-CO2
  • Catalog:
    6875
  • Application:
    WB, ICC
  • Species Reactivity:
    H
Information
Product Name MT-CO2 Recombinant Rabbit mAb
Aliases MT-CO2; Mitochondrially Encoded Cytochrome C Oxidase II; COX2; MTCO2; COII; CO2; Cytochrome C Oxidase Polypeptide II; Cytochrome C Oxidase Subunit II; Cytochrome C Oxidase Subunit 2; Cytochrome C Oxidase II; EC 7.1.1.9; EC 1.9.3.1; COXII
Background

Gene Name: MT-CO2

NCBI Gene Entry: 4513

UniProt Entry: P00403

Application Information

Molecular Weight: Predicted, 26 kDa; observed, 20 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB1125

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human MTCO2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using MT-CO2 antibody (Cat#6875). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MT-CO2 antibody (Cat#6875, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with MT-CO2 antibody (Cat#6875, 1:1,000). Nuclei were stained blue with DAPI; MT-CO2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using MT-CO2 antibody (Cat#6875). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MT-CO2 antibody (Cat#6875, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with MT-CO2 antibody (Cat#6875, 1:1,000). Nuclei were stained blue with DAPI; MT-CO2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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