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Home>Product>Primary Antibody

ERG Recombinant Rabbit mAb

#H1132

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Western blotting analysis using ERG antibody (Cat#H1132). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ERG antibody (Cat#H1132, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Immunocytochemical staining shows positive staining in paraffin-embedded human endometrial cancer. ERG antibody (Cat#H1132) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. ERG antibody (Cat#H1132) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human tonsil. ERG antibody (Cat#H1132) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human spleen. ERG antibody (Cat#H1132) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human kidney. ERG antibody (Cat#H1132) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ERG
  • Catalog：
    H1132
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name ERG Recombinant Rabbit mAb
Aliases ERG; ETS Transcription Factor ERG; Erg-3; P55; Transcriptional Regulator ERG (Transforming Protein ERG); V-Ets Avian Erythroblastosis Virus E26 Oncogene Related; V-Ets Avian Erythroblastosis Virus E26 Oncogene Homolog; V-Ets Erythroblastosis Virus E26 Oncogene Like; Transcriptional Regulator ERG; V-Ets Erythroblastosis Virus E26 Oncogene Homolog; TMPRSS2-ERG Prostate Cancer Specific; ERG, ETS Transcription Factor; Transforming Protein ERG; FUS/ERG Fusion Protein; TMPRSS2/ERG Fusion; ETS-Related Gene; Ets-Related; LMPHM14
Background

Gene Name: ERG

NCBI Gene Entry: 2078

UniProt Entry: P11308

Application Information

Molecular Weight: Predicted, 54 kDa; observed, 50kDa

Clonality: Recombinant Monoclonal Antibody

Clone ID: SDT-190-65

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human ERG
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:200-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ERG antibody (Cat#H1132). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ERG antibody (Cat#H1132, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining shows positive staining in paraffin-embedded human endometrial cancer. ERG antibody (Cat#H1132) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. ERG antibody (Cat#H1132) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human tonsil. ERG antibody (Cat#H1132) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human spleen. ERG antibody (Cat#H1132) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human kidney. ERG antibody (Cat#H1132) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ERG
  • Catalog：
    H1132
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using ERG antibody (Cat#H1132). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ERG antibody (Cat#H1132, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Immunocytochemical staining shows positive staining in paraffin-embedded human endometrial cancer. ERG antibody (Cat#H1132) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. ERG antibody (Cat#H1132) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human tonsil. ERG antibody (Cat#H1132) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human spleen. ERG antibody (Cat#H1132) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human kidney. ERG antibody (Cat#H1132) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
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