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  • Primary Antibody
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Home>Product>Primary Antibody

Phospho-eIF4EBP1/2/3 (T46+T46+T32) Recombinant Rabbit mAb

#6197

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Western blotting analysis using phospho-eIF4EBP1/2/3 (T46+T46+T32) antibody (Cat#6197). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-eIF4EBP1/2/3 (T46+T46+T32) antibody (Cat#6197, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Phospho-eIF4EBP1/2/3 (T46+T46+T32) expression in C2C12 cells using Phospho-eIF4EBP1/2/3 (T46+T46+T32) antibody (Cat#6197, 1:2,000). Green, isotype control; red, Phospho-eIF4EBP1/2/3 (T46+T46+T32).
Immunocytochemical staining of C2C12 cells with Phospho-eIF4EBP1/2/3 (T46+T46+T32) antibody (Cat#6197, 1:1,000). Nuclei were stained blue with DAPI; Phospho-eIF4EBP1/2/3 (T46+T46+T32) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    EIF4EBP1/2/3
  • Catalog：
    6197
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospho-eIF4EBP1/2/3 (T46+T46+T32) Recombinant Rabbit mAb
Aliases EIF4EBP1/2/3; Eukaryotic Translation Initiation Factor 4E Binding Protein 1/2/3; PHAS-I; 4E-BP1/2/3; Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1/2/3; Eukaryotic Translation Initiation Factor 4E-Binding Protein 1/2/3; EIF4E-Binding Protein 1/2/3; 4EBP1/2/3; BP-1/2/3
Background

Gene Name: EIF4EBP1/2/3

NCBI Gene Entry: 1978 , 1979 , 8637

UniProt Entry: Q13541 , Q13542 , O60516

Application Information

Molecular Weight: Predicted, 13 kDa, observed, 14,17 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB7565

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-eIF4EBP1/2/3 (T46+T46+T32)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using phospho-eIF4EBP1/2/3 (T46+T46+T32) antibody (Cat#6197). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-eIF4EBP1/2/3 (T46+T46+T32) antibody (Cat#6197, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of Phospho-eIF4EBP1/2/3 (T46+T46+T32) expression in C2C12 cells using Phospho-eIF4EBP1/2/3 (T46+T46+T32) antibody (Cat#6197, 1:2,000). Green, isotype control; red, Phospho-eIF4EBP1/2/3 (T46+T46+T32).
  • Immunocytochemical staining of C2C12 cells with Phospho-eIF4EBP1/2/3 (T46+T46+T32) antibody (Cat#6197, 1:1,000). Nuclei were stained blue with DAPI; Phospho-eIF4EBP1/2/3 (T46+T46+T32) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    EIF4EBP1/2/3
  • Catalog：
    6197
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using phospho-eIF4EBP1/2/3 (T46+T46+T32) antibody (Cat#6197). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-eIF4EBP1/2/3 (T46+T46+T32) antibody (Cat#6197, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Phospho-eIF4EBP1/2/3 (T46+T46+T32) expression in C2C12 cells using Phospho-eIF4EBP1/2/3 (T46+T46+T32) antibody (Cat#6197, 1:2,000). Green, isotype control; red, Phospho-eIF4EBP1/2/3 (T46+T46+T32).
Immunocytochemical staining of C2C12 cells with Phospho-eIF4EBP1/2/3 (T46+T46+T32) antibody (Cat#6197, 1:1,000). Nuclei were stained blue with DAPI; Phospho-eIF4EBP1/2/3 (T46+T46+T32) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  139. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-helper/src/Collection.php ( 16.47 KB )
  140. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/Raw.php ( 1.22 KB )
  141. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/View.php ( 1.70 KB )
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  143. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/view/driver/Php.php ( 6.44 KB )
  144. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/contract/TemplateHandlerInterface.php ( 1.60 KB )
  145. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public.php ( 3.56 KB )
  146. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_param.php ( 0.75 KB )
  147. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Request.php ( 9.20 KB )
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  149. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
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  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
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  1. CONNECT:[ UseTime:0.000375s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000672s ]
  3. SELECT * FROM `goods` WHERE `code` = '6197' LIMIT 1 [ RunTime:0.000437s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000413s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000297s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000374s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 7008 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000324s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000447s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 7008 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000848s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000433s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000274s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.060544s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (6899,6205,8243,3683,7635,7296,9098,9010,4820,5255) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000724s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000447s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000215s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000534s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000272s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000395s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000392s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000228s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000212s ]
0.117687s