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UHRF1 Recombinant Rabbit mAb

#6892

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Recombinant Antibody
Western blotting analysis using UHRF1 antibody (Cat#6892). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UHRF1 antibody (Cat#6892, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with UHRF1 antibody (Cat#6892, 1:1,000). Nuclei were stained blue with DAPI; UHRF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    UHRF1
  • Catalog:
    6892
  • Application:
    WB, ICC
  • Species Reactivity:
    H
Information
Product Name UHRF1 Recombinant Rabbit mAb
Aliases UHRF1; Ubiquitin Like With PHD And Ring Finger Domains 1; ICBP90; RNF106; TDRD22; Np95; Ubiquitin-Like PHD And RING Finger Domain-Containing Protein 1; Inverted CCAAT Box-Binding Protein Of 90 KDa; RING-Type E3 Ubiquitin Transferase UHRF1; Inverted CCAAT Box Binding Protein 90; E3 Ubiquitin-Protein Ligase UHRF1; Nuclear Zinc Finger Protein Np95; Transcription Factor ICBP90; RING Finger Protein 106; Nuclear Protein 95; FLJ21925; HUHRF1; HuNp95; Ubiquitin-Like-Containing PHD And RING Finger Domains Protein 1; Nuclear Phosphoprotein 95; EC 2.3.2.27; EC 6.3.2; HNP95; HNp95; NP95
Background

Gene Name: UHRF1

NCBI Gene Entry: 29128

UniProt Entry: Q96T88

Application Information

Molecular Weight: Predicted, 90 kDa; observed, 100 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB1210

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen Recombinant protein of human UHRF1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using UHRF1 antibody (Cat#6892). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UHRF1 antibody (Cat#6892, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with UHRF1 antibody (Cat#6892, 1:1,000). Nuclei were stained blue with DAPI; UHRF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using UHRF1 antibody (Cat#6892). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UHRF1 antibody (Cat#6892, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with UHRF1 antibody (Cat#6892, 1:1,000). Nuclei were stained blue with DAPI; UHRF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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