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Home>Product>Primary Antibody

KO-Validated HLA-C Mouse mAb

#71122

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Knockout Validated
Western blotting analysis using HLA-C antibody (Cat#71122). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLA-C antibody (Cat#71122, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using HLA-C antibody (Cat#71122). HLA-C expression in wild type (WT) and HLA-C knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HLA-C antibody (Cat#71122, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of HLA-C expression in HAP-1 cells using HLA-C antibody (Cat#71122, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, HLA-C.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    HLA-C
  • Catalog：
    71122
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name KO-Validated HLA-C Mouse mAb
Aliases HLA-C; Major Histocompatibility Complex, Class I, C; HLA Class I Histocompatibility Antigen, C Alpha Chain; HLA-JY3; D6S204; PSORS1; HLAC; Major Histocompatibility Antigen HLA-C; MHC Class I Antigen Heavy Chain HLA-C; Human Leukocyte Antigen-C Alpha Chain; Psoriasis Susceptibility 1; Human Leukocyte Antigen C; HLA-C Antigen; HLA-Cw; HLC-C; MHC
Background

Gene Name: HLA-C

NCBI Gene Entry: 3107

UniProt Entry: P10321

Application Information

Molecular Weight: Predicted, 41 kDa; observed, 40 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB8545

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human HLA-C
Isotype Mouse IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using HLA-C antibody (Cat#71122). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLA-C antibody (Cat#71122, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using HLA-C antibody (Cat#71122). HLA-C expression in wild type (WT) and HLA-C knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HLA-C antibody (Cat#71122, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of HLA-C expression in HAP-1 cells using HLA-C antibody (Cat#71122, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, HLA-C.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HLA-C
  • Catalog：
    71122
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using HLA-C antibody (Cat#71122). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLA-C antibody (Cat#71122, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using HLA-C antibody (Cat#71122). HLA-C expression in wild type (WT) and HLA-C knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HLA-C antibody (Cat#71122, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of HLA-C expression in HAP-1 cells using HLA-C antibody (Cat#71122, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, HLA-C.
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