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Home>Product>Primary Antibody

KO-Validated Phospho-MSK1 (S376) Recombinant Rabbit mAb

#71200

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Recombinant Antibody
Knockout Validated
Western blotting analysis using phospho-MSK1 (S376) antibody (Cat#71200). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-MSK1 (S376) antibody (Cat#71200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using phospho-MSK1 (S376) antibody (Cat#71200). Phospho-MSK1 (S376) expression in wild-type (WT) and RPS6KA5 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-MSK1 (S376) antibody (Cat#71200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using phospho-MSK1 (S376) antibody (Cat#71200, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded human melanoma using phospho-MSK1 (S376) antibody (Cat#71200, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of H9c2 cells with Phospho-MSK1 (S376) antibody (Cat#71200, 1:1,000). Nuclei were stained blue with DAPI; Phospho-MSK1 (S376) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Phospho-MSK1 (S376) expression in H9c2 cells using Phospho-MSK1 (S376) antibody (Cat#71200, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-MSK1 (S376).
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  • Gene Symbol：
    RPS6KA5
  • Catalog：
    71200
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KO-Validated Phospho-MSK1 (S376) Recombinant Rabbit mAb
Aliases RPS6KA5; Ribosomal Protein S6 Kinase A5; MSK1; RLPK; Nuclear Mitogen- And Stress-Activated Protein Kinase 1; 90 KDa Ribosomal Protein S6 Kinase 5; Ribosomal Protein S6 Kinase Alpha-5; RSK-Like Protein Kinase; S6K-Alpha-5; EC 2.7.11.1; RSKL; Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5; Ribosomal Protein S6 Kinase, 90kD, Polypeptide 5; EC 2.7.11; MSPK1
Background

Gene Name: RPS6KA5

NCBI Gene Entry: 9252

UniProt Entry: O75582

Application Information

Molecular Weight: Predicted, 90 kDa; observed, 90 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2625

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human Phospho-MSK1 (S376)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using phospho-MSK1 (S376) antibody (Cat#71200). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-MSK1 (S376) antibody (Cat#71200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using phospho-MSK1 (S376) antibody (Cat#71200). Phospho-MSK1 (S376) expression in wild-type (WT) and RPS6KA5 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-MSK1 (S376) antibody (Cat#71200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using phospho-MSK1 (S376) antibody (Cat#71200, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded human melanoma using phospho-MSK1 (S376) antibody (Cat#71200, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of H9c2 cells with Phospho-MSK1 (S376) antibody (Cat#71200, 1:1,000). Nuclei were stained blue with DAPI; Phospho-MSK1 (S376) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of Phospho-MSK1 (S376) expression in H9c2 cells using Phospho-MSK1 (S376) antibody (Cat#71200, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-MSK1 (S376).
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RPS6KA5
  • Catalog：
    71200
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using phospho-MSK1 (S376) antibody (Cat#71200). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-MSK1 (S376) antibody (Cat#71200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using phospho-MSK1 (S376) antibody (Cat#71200). Phospho-MSK1 (S376) expression in wild-type (WT) and RPS6KA5 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-MSK1 (S376) antibody (Cat#71200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using phospho-MSK1 (S376) antibody (Cat#71200, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded human melanoma using phospho-MSK1 (S376) antibody (Cat#71200, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of H9c2 cells with Phospho-MSK1 (S376) antibody (Cat#71200, 1:1,000). Nuclei were stained blue with DAPI; Phospho-MSK1 (S376) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Phospho-MSK1 (S376) expression in H9c2 cells using Phospho-MSK1 (S376) antibody (Cat#71200, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-MSK1 (S376).
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