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KD-Validated FIS1 Recombinant Rabbit mAb

#62040

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using FIS1 antibody (Cat#62040). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FIS1 antibody (Cat#62040, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using FIS1 antibody (Cat#62040). FIS1 expression in wild-type (WT) and FIS1 shRNA knockdown (KD) HeLa cells with 15 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FIS1 antibody (Cat#62040, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    FIS1
  • Catalog:
    62040
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated FIS1 Recombinant Rabbit mAb
Aliases FIS1; Fission, Mitochondrial 1; H_NH0132A01.6; CGI-135; TTC11; Tetratricopeptide Repeat Protein 11; Tetratricopeptide Repeat Domain 11; Mitochondrial Fission 1 Protein; Mitochondrial Fission Molecule; TPR Repeat Protein 11; FIS1 Homolog; HFis1; Fission 1 (Mitochondrial Outer Membrane) Homolog (S. Cerevisiae); Fission 1 (Mitochondrial Outer Membrane) Homolog (Yeast); Fission 1 (Mitochondrial Outer Membrane) Homolog; CGI-135 Protein
Background

Gene Name: FIS1

NCBI Gene Entry: 51024

UniProt Entry: Q9Y3D6

Application Information

Molecular Weight: Predicted, 17 kDa; observed, 15 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB8040

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human TTC11
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using FIS1 antibody (Cat#62040). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FIS1 antibody (Cat#62040, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using FIS1 antibody (Cat#62040). FIS1 expression in wild-type (WT) and FIS1 shRNA knockdown (KD) HeLa cells with 15 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FIS1 antibody (Cat#62040, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using FIS1 antibody (Cat#62040). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FIS1 antibody (Cat#62040, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using FIS1 antibody (Cat#62040). FIS1 expression in wild-type (WT) and FIS1 shRNA knockdown (KD) HeLa cells with 15 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FIS1 antibody (Cat#62040, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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