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LPCAT1 Recombinant Rabbit mAb

#6039

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Recombinant Antibody
Western blotting analysis using LPCAT1 antibody (Cat#6039). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LPCAT1 antibody (Cat#6039, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using LPCAT1 antibody (Cat#6039, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    LPCAT1
  • Catalog:
    6039
  • Application:
    WB, IHC-P
  • Species Reactivity:
    H, R
Information
Product Name LPCAT1 Recombinant Rabbit mAb
Aliases LPCAT1; Lysophosphatidylcholine Acyltransferase 1; AGPAT10; AGPAT9; LPLAT8; AYTL2; Acetyl-CoA:Lyso-Platelet-Activating Factor Acetyltransferase; 1-Alkenylglycerophosphocholine O-Acyltransferase; 1-Alkylglycerophosphocholine O-Acetyltransferase; 1-Acylglycerophosphocholine O-Acyltransferase; 1-Acylglycerol-3-Phosphate O-Acyltransferase; Phosphonoformate Immuno-Associated Protein 3; Acetyl-CoA:Lyso-PAF Acetyltransferase; Lysophospholipid Acyltransferase 8; Lyso-PAF Acetyltransferase; LysoPC Acyltransferase 1; Acyltransferase-Like; LPC Acyltransferase 1; LysoPAFAT; FLJ12443; LPCAT-1; PFAAP3; Acyltransferase Like 2; EC 2.3.1.23; EC 2.3.1.51; EC 2.3.1.25; EC 2.3.1.67; Lpcat
Background

Gene Name: LPCAT1

NCBI Gene Entry: 79888

UniProt Entry: Q8NF37

Application Information

Molecular Weight: Predicted, 59 kDa; observed, 55 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB6520

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)

Immunogen Recombinant protein of human LPCAT1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunohistochemistry-Paraffin (IHC-P): 1:500-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using LPCAT1 antibody (Cat#6039). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LPCAT1 antibody (Cat#6039, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using LPCAT1 antibody (Cat#6039, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using LPCAT1 antibody (Cat#6039). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LPCAT1 antibody (Cat#6039, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using LPCAT1 antibody (Cat#6039, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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