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KD-Validated SMC1A Mouse mAb

#62175

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shRNA Knockdown Validated
Western blotting analysis using SMC1A antibody (Cat#62175). SMC1A expression in wild-type (WT) and SMC1A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SMC1A antibody (Cat#62175, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    SMC1A
  • Catalog:
    62175
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated SMC1A Mouse mAb
Aliases SMC1A; Structural Maintenance Of Chromosomes 1A; DXS423E; SB1.8; KIAA0178; SMC1L1; Smcb; SMC1 (Structural Maintenance Of Chromosomes 1, Yeast)-Like 1; Structural Maintenance Of Chromosomes Protein 1A; SMC Protein 1A; SMC-1-Alpha; SMC1; SMC1 Structural Maintenance Of Chromosomes 1-Like 1 (Yeast); Epididymis Secretory Sperm Binding Protein; Segregation Of Mitotic Chromosomes 1; SMC1alpha; EIEE85; SMC-1A; CDLS2; DEE85; Sb1.8
Background

Gene Name: SMC1A

NCBI Gene Entry: 8243

UniProt Entry: Q14683

Application Information

Molecular Weight: Predicted, 143 kDa; observed, 150 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB1680

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human SMC1A
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using SMC1A antibody (Cat#62175). SMC1A expression in wild-type (WT) and SMC1A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SMC1A antibody (Cat#62175, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using SMC1A antibody (Cat#62175). SMC1A expression in wild-type (WT) and SMC1A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SMC1A antibody (Cat#62175, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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