Western blotting analysis using PTPN6 antibody (Cat#5137). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PTPN6 antibody (Cat#5137, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PTPN6 expression in HaCaT cells using PTPN6 antibody (Cat#5137, 1:2,000). Green, isotype control; red, PTPN6.
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using PTPN6 antibody (Cat#5137, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Western blotting analysis using PTPN6 antibody (Cat#5137). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PTPN6 antibody (Cat#5137, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PTPN6 expression in HaCaT cells using PTPN6 antibody (Cat#5137, 1:2,000). Green, isotype control; red, PTPN6.
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using PTPN6 antibody (Cat#5137, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Western blotting analysis using PTPN6 antibody (Cat#5137). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PTPN6 antibody (Cat#5137, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PTPN6 expression in HaCaT cells using PTPN6 antibody (Cat#5137, 1:2,000). Green, isotype control; red, PTPN6.
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using PTPN6 antibody (Cat#5137, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000778s ]
SELECT * FROM `goods` WHERE `code` = '5137' LIMIT 1 [ RunTime:0.000514s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000491s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000379s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000457s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6294 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000348s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001171s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6294 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000311s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000455s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000614s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.143304s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4096,10238,9994,7472,10341,3974,4091,8042,4146,8980) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000955s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000549s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000699s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.001300s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.001779s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000366s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000501s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000278s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.001510s ]
