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Home>Product>Primary Antibody

KO-Validated JUN Rabbit pAb

#71275

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Knockout Validated
Western blotting analysis using JUN antibody (Cat#71275). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with JUN antibody (Cat#71275, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using JUN antibody (Cat#71275). JUN expression in wild-type (WT) and JUN knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with JUN antibody (Cat#71275, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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  • Gene Symbol：
    JUN
  • Catalog：
    71275
  • Application：
    WB
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KO-Validated JUN Rabbit pAb
Aliases JUN; Jun Proto-Oncogene, AP-1 Transcription Factor Subunit; V-Jun Avian Sarcoma Virus 17 Oncogene Homolog 2; C-Jun; AP-1; Transcription Factor AP-1 Subunit Jun; Transcription Factor Jun; Proto-Oncogene C-Jun; Activator Protein 1; Jun Oncogene; AP1; P39; V-Jun Sarcoma Virus 17 Oncogene Homolog; Jun Activation Domain Binding Protein; Enhancer-Binding Protein AP1; Transcription Factor AP-1; Proto-Oncogene CJun; CJUN
Background

Gene Name: JUN

NCBI Gene Entry: 3725

UniProt Entry: P05412

Application Information

Molecular Weight: Predicted, 36 kDa; observed, 39 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human JUN
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using JUN antibody (Cat#71275). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with JUN antibody (Cat#71275, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using JUN antibody (Cat#71275). JUN expression in wild-type (WT) and JUN knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with JUN antibody (Cat#71275, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    JUN
  • Catalog：
    71275
  • Application：
    WB
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using JUN antibody (Cat#71275). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with JUN antibody (Cat#71275, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using JUN antibody (Cat#71275). JUN expression in wild-type (WT) and JUN knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with JUN antibody (Cat#71275, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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