Western blotting analysis using CCAR2 antibody (Cat#71309). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CCAR2 antibody (Cat#71309, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CCAR2 antibody (Cat#71309). CCAR2 expression in wild-type (WT) and CCAR2 knockout (KO) 293T cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with CCAR2 antibody (Cat#71309, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
CCAR2; Cell Cycle And Apoptosis Regulator 2; KIAA1967; DBC-1; NET35; DBC1; Cell Division Cycle And Apoptosis Regulator Protein 2; Cell Cycle And Apoptosis Regulator Protein 2; Deleted In Breast Cancer 1; P30 DBC; Deleted In Breast Cancer Gene 1 Protein; DBIRD Complex Subunit KIAA1967; P30DBC; DBC.1
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000
Note
This product is for research use only.
Data
Western blotting analysis using CCAR2 antibody (Cat#71309). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CCAR2 antibody (Cat#71309, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CCAR2 antibody (Cat#71309). CCAR2 expression in wild-type (WT) and CCAR2 knockout (KO) 293T cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with CCAR2 antibody (Cat#71309, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CCAR2 antibody (Cat#71309). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CCAR2 antibody (Cat#71309, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CCAR2 antibody (Cat#71309). CCAR2 expression in wild-type (WT) and CCAR2 knockout (KO) 293T cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with CCAR2 antibody (Cat#71309, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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