Cart

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News

Sign In/Register

Cart
  • Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products
Home>Product>Primary Antibody

KO-Validated SIRT1 Recombinant Rabbit mAb

#71283

Click to copy product information

Return to List

Recombinant Antibody
Knockout Validated
Western blotting analysis using SIRT1 antibody (Cat#71283). SIRT1 expression in wild type (WT) and SIRT1 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SIRT1 antibody (Cat#71283, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using SIRT1 antibody (Cat#71283, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SIRT1
  • Catalog：
    71283
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name KO-Validated SIRT1 Recombinant Rabbit mAb
Aliases SIRT1; Sirtuin 1; SIR2L1; NAD-Dependent Protein Deacetylase Sirtuin-1; NAD-Dependent Protein; Deacylase Sirtuin-1; Regulatory Protein SIR2 Homolog 1; SIR2-Like Protein 1; Sirtuin (Silent Mating Type Information Regulation 2, S. Cerevisiae, Homolog) 1; Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 1 (S. Cerevisiae); Sirtuin Type 1; EC 2.3.1.286; EC 2.3.1.-; SIR2alpha; HSIRT1; HSIR2; SIR2
Background

Gene Name: SIRT1

NCBI Gene Entry: 23411

UniProt Entry: Q96EB6

Application Information

Molecular Weight: Predicted, 82 kDa; observed, 120 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB6190

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human SIRT1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using SIRT1 antibody (Cat#71283). SIRT1 expression in wild type (WT) and SIRT1 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SIRT1 antibody (Cat#71283, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using SIRT1 antibody (Cat#71283, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SIRT1
  • Catalog：
    71283
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using SIRT1 antibody (Cat#71283). SIRT1 expression in wild type (WT) and SIRT1 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SIRT1 antibody (Cat#71283, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using SIRT1 antibody (Cat#71283, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$
View cart Empty cart
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.