Western blotting analysis using NFKB inhibitor alpha antibody (Cat#61781). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NFKB inhibitor alpha antibody (Cat#61781, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NFKB inhibitor alpha antibody (Cat#61781). NFKB inhibitor alpha expression in wild type (WT) and NFKB inhibitor alpha (NFKBIA) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NFKB inhibitor alpha antibody (Cat#61781, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with NFKB inhibitor alpha antibody (Cat#61781, 1:1,000). Nuclei were stained blue with DAPI; was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using NFKB inhibitor alpha antibody (Cat#61781, 1:1,000), Top panel: wild-type (WT); Bottom panal: NFKB inhibitor alpha shRNA knockdown (KD). Nuclei were stained blue with DAPI; NFKB inhibitor alpha was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
