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Home>Product>Primary Antibody

KO-Validated Phospho-POLR2A (S5) Recombinant Rabbit mAb

#71289

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Recombinant Antibody
Knockout Validated
Western blotting analysis using Phospho-POLR2A (S5) antibody (Cat#71289). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-POLR2A (S5) antibody (Cat#71289, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Western blotting analysis using phospho-POLR2A (S5) antibody (Cat#71289). Phospho-POLR2A (S5) expression in wild type (WT) and POLR2A knockout (KO) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-POLR2A (S5) antibody (Cat#71289, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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  • Gene Symbol：
    POLR2A
  • Catalog：
    71289
  • Application：
    WB
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KO-Validated Phospho-POLR2A (S5) Recombinant Rabbit mAb
Aliases RNA Polymerase II Subunit A; RNA Polymerase II Subunit B1; POLRA; POLR2; RPB1; DNA-Directed RNA Polymerase II Largest Subunit, RNA Polymerase II 220 Kd Subunit; Polymerase (RNA) II (DNA Directed) Polypeptide A, 220kDa; DNA-Directed RNA Polymerase III Largest Subunit; DNA-Directed RNA Polymerase II Subunit RPB1; RNA-Directed RNA Polymerase II Subunit RPB1; DNA-Directed RNA Polymerase II Subunit A; EC 2.7.7.6; Polymerase (RNA) II Subunit A; EC 2.7.7.48; HRPB220; NEDHIB; RpIILS; HsRPB1; RPBh1; RPOL2; RPO2
Background

Gene Name: POLR2A

NCBI Gene Entry: 5430

UniProt Entry: P24928

Application Information

Molecular Weight: Predicted, 217 kDa; observed, 270 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB9470

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human Phospho-POLR2A (S5)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using Phospho-POLR2A (S5) antibody (Cat#71289). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-POLR2A (S5) antibody (Cat#71289, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
  • Western blotting analysis using phospho-POLR2A (S5) antibody (Cat#71289). Phospho-POLR2A (S5) expression in wild type (WT) and POLR2A knockout (KO) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-POLR2A (S5) antibody (Cat#71289, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    POLR2A
  • Catalog：
    71289
  • Application：
    WB
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Phospho-POLR2A (S5) antibody (Cat#71289). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-POLR2A (S5) antibody (Cat#71289, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Western blotting analysis using phospho-POLR2A (S5) antibody (Cat#71289). Phospho-POLR2A (S5) expression in wild type (WT) and POLR2A knockout (KO) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-POLR2A (S5) antibody (Cat#71289, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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