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Home>Product>Primary Antibody

BCL11 Transcription Factor B Recombinant Rabbit mAb

#8141

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Recombinant Antibody
Western blotting analysis using BCL11 transcription factor B antibody (Cat#8141). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCL11 transcription factor B antibody (Cat#8141, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with BCL11 transcription factor B antibody (Cat#8141, 1:1,000). Nuclei were stained blue with DAPI; BCL11 transcription factor B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of BCL11 transcription factor B expression in HepG2 cells using BCL11 transcription factor B antibody (Cat#8141, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, BCL11 transcription factor B.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    BCL11B
  • Catalog：
    8141
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name BCL11 Transcription Factor B Recombinant Rabbit mAb
Aliases BCL11 Transcription Factor B; CTIP2; BAF Chromatin Remodeling Complex Subunit BCL11B; HRIT1-Alpha; SMARCM2; CTIP-2; Radiation-Induced Tumor Suppressor Gene 1 Protein; B-Cell CLL/Lymphoma 11B (Zinc Finger Protein); BCL11B, BAF Complex Component; COUP-TF-Interacting Protein 2; B-Cell Lymphoma/Leukemia 11B; B Cell CLL/Lymphoma 11B; ZNF856B; BCL-11B; HRit1; RIT1; B-Cell CLL/Lymphoma 11B/T-Cell Receptor Delta Constant Region Fusion Protein; Zinc Finger Protein HRit1 Alpha; B-Cell Lymphoma/Leukaemia 11B; B-Cell CLL/Lymphoma 11B; BCL11B/TRDC Fusion; ATL1-Alpha; ATL1-Delta; ATL1-Gamma; ATL1-Beta; IDDFSTA; IMD49; ATL1
Background

Gene Name: BCL11B

NCBI Gene Entry: 64919

UniProt Entry: Q9C0K0

Application Information

Molecular Weight: Predicted, 96 kDa; observed, 140 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1630

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Ctip2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using BCL11 transcription factor B antibody (Cat#8141). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCL11 transcription factor B antibody (Cat#8141, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with BCL11 transcription factor B antibody (Cat#8141, 1:1,000). Nuclei were stained blue with DAPI; BCL11 transcription factor B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of BCL11 transcription factor B expression in HepG2 cells using BCL11 transcription factor B antibody (Cat#8141, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, BCL11 transcription factor B.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    BCL11B
  • Catalog：
    8141
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using BCL11 transcription factor B antibody (Cat#8141). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCL11 transcription factor B antibody (Cat#8141, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with BCL11 transcription factor B antibody (Cat#8141, 1:1,000). Nuclei were stained blue with DAPI; BCL11 transcription factor B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of BCL11 transcription factor B expression in HepG2 cells using BCL11 transcription factor B antibody (Cat#8141, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, BCL11 transcription factor B.
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