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Home>Product>Primary Antibody

Hypoxia Inducible Factor 1 Subunit Alpha Recombinant Rabbit mAb

#9321

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Recombinant Antibody
Western blotting analysis using hypoxia inducible factor 1 subunit alpha antibody (Cat#9321). Total cell lysates (30 μg) from various cell lines untreated or treated with cobalt chloride (250 μM, 6 hr) were loaded and separated by SDS-PAGE. The blot was incubated with hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat.#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with Hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:1,000). Nuclei were stained blue with DAPI; Hypoxia inducible factor 1 subunit alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of hypoxia inducible factor 1 subunit alpha expression in HT-1080 cells using hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, hypoxia inducible factor 1 subunit alpha.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HIF1A
  • Catalog：
    9321
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Hypoxia Inducible Factor 1 Subunit Alpha Recombinant Rabbit mAb
Aliases Hypoxia Inducible Factor 1 Subunit Alpha; Hypoxia Inducible Factor 1, Alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) ; Class E Basic Helix-Loop-Helix Protein 78; Basic-Helix-Loop-Helix-PAS Protein MOP1; Hypoxia-Inducible Factor 1-Alpha; PAS Domain-Containing Protein 8; Member Of PAS Protein 1; HIF-1-Alpha; BHLHe78; PASD8; MOP1; Hypoxia-Inducible Factor 1 Alpha Isoform I.3; Hypoxia Inducible Factor 1 Alpha Subunit; Hypoxia-Inducible Factor1alpha; Member Of PAS Superfamily 1; ARNT Interacting Protein; ARNT-Interacting Protein; HIF-1alpha; HIF1-ALPHA; HIF1-Alpha; BHLHE78; HIF-1A; HIF1
Background

Gene Name: HIF1A

NCBI Gene Entry: 3091

UniProt Entry: Q16665

Application Information

Molecular Weight: Predicted, 93 kDa; observed, 120 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1625

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human HIF-1-alpha
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using hypoxia inducible factor 1 subunit alpha antibody (Cat#9321). Total cell lysates (30 μg) from various cell lines untreated or treated with cobalt chloride (250 μM, 6 hr) were loaded and separated by SDS-PAGE. The blot was incubated with hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat.#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HT-1080 cells with Hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:1,000). Nuclei were stained blue with DAPI; Hypoxia inducible factor 1 subunit alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of hypoxia inducible factor 1 subunit alpha expression in HT-1080 cells using hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, hypoxia inducible factor 1 subunit alpha.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HIF1A
  • Catalog：
    9321
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using hypoxia inducible factor 1 subunit alpha antibody (Cat#9321). Total cell lysates (30 μg) from various cell lines untreated or treated with cobalt chloride (250 μM, 6 hr) were loaded and separated by SDS-PAGE. The blot was incubated with hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat.#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with Hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:1,000). Nuclei were stained blue with DAPI; Hypoxia inducible factor 1 subunit alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of hypoxia inducible factor 1 subunit alpha expression in HT-1080 cells using hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, hypoxia inducible factor 1 subunit alpha.
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