Western blotting analysis using RNA binding fox-1 homolog 3 antibody (Cat#H1138). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using RNA binding fox-1 homolog 3 antibody (Cat#H1138). RNA binding fox-1 homolog 3 expression in wild-type (WT) and RNA binding fox-1 homolog 3 (RBFOX3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:500). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining shows negative staining in paraffin-embedded rat liver. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded rat cerebral cortex. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded mouse liver. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded mouse cerebral cortex. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human baldder cancer. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human glioma. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human cardiac muscle. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunohistochemistry was performed on paraffin-embedded mouse brain using RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:500). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining shows positive staining in paraffin-embedded human cerebellum. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Flow cytometric analysis of RNA binding fox-1 homolog 3 expression in A549 cells using RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:2,000). Green, isotype control; red, RNA binding fox-1 homolog 3.
