Cart

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News

Sign In/Register

Cart
  • Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products
Home>Product>Primary Antibody

KD-Validated Phospho-mTOR (S2448) Rabbit mAb

#R0009

一键复制产品信息

Return to List

Knockdown Validated
Western blotting analysis using anti-phospho-mTOR (Ser2448) antibody (Cat#R0009). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-mTOR (Ser2448) antibody (Cat#R0009, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-phospho-mTOR (Ser2448) antibody (Cat#R0009). Phospho-mTOR (Ser2448) expression in wild-type (WT) and MTOR shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-mTOR (Ser2448) antibody (Cat#R0009, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with anti-Phospho-mTOR (Ser2448) antibody (Cat#R0009, 1:1,000). Nuclei were stained blue with DAPI; Phospho-mTOR (Ser2448) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using anti-Phospho-mTOR (Ser2448) antibody (Cat#R0009, 1:1,000), Top panel: wild-type (WT); Bottom panal: Phospho-mTOR (Ser2448) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-mTOR (Ser2448) was stained magenta with Alexa Fluor® 647. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-mTOR (Ser2448) expression in HepG2 cells using anti-Phospho-mTOR (Ser2448) antibody (Cat#R0009, 1:2,000). Green, isotype control; red, Phospho-mTOR (Ser2448).
Validation of mTOR knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Phospho-mTOR (Ser2448) antibody (Cat#R0009, 1:2,000) and analyzed using BD flow cytometer.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MTOR
  • Catalog：
    R0009
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated Phospho-mTOR (S2448) Rabbit mAb
Aliases MTOR; Mechanistic Target Of Rapamycin Kinase; RAFT1; RAPT1; Rapamycin And FKBP12 Target 1; Mammalian Target Of Rapamycin; FRAP1; FRAP2; FRAP; FK506-Binding Protein 12-Rapamycin Complex-Associated Protein 1; Mechanistic Target Of Rapamycin (Serine/Threonine Kinase); FK506 Binding Protein 12-Rapamycin Associated Protein 2; FKBP12-Rapamycin Complex-Associated Protein 1; Serine/Threonine-Protein Kinase MTOR; Rapamycin Associated Protein FRAP2; FKBP-Rapamycin Associated Protein; Mechanistic Target Of Rapamycin; Rapamycin Target Protein 1; FLJ44809; DJ576K7.1 (FK506 Binding Protein 12-Rapamycin Associated Protein 1); FK506 Binding Protein 12-Rapamycin Associated Protein 1; FKBP12-Rapamycin Complex-Associated Protein; Rapamycin Target Protein; EC 2.7.11.1; MTOR; SKS
Background

Gene Name: MTOR

NCBI Gene Entry: 2475

UniProt Entry: P42345

Application Information

Molecular Weight: Predicted, 289 kDa; observed, 250 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: S-705-7

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-mTOR (S2448)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using anti-phospho-mTOR (Ser2448) antibody (Cat#R0009). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-mTOR (Ser2448) antibody (Cat#R0009, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-phospho-mTOR (Ser2448) antibody (Cat#R0009). Phospho-mTOR (Ser2448) expression in wild-type (WT) and MTOR shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-mTOR (Ser2448) antibody (Cat#R0009, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with anti-Phospho-mTOR (Ser2448) antibody (Cat#R0009, 1:1,000). Nuclei were stained blue with DAPI; Phospho-mTOR (Ser2448) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Immunocytochemical staining of HeLa cells using anti-Phospho-mTOR (Ser2448) antibody (Cat#R0009, 1:1,000), Top panel: wild-type (WT); Bottom panal: Phospho-mTOR (Ser2448) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-mTOR (Ser2448) was stained magenta with Alexa Fluor® 647. Scale bar: 20 μm.
  • Flow cytometric analysis of Phospho-mTOR (Ser2448) expression in HepG2 cells using anti-Phospho-mTOR (Ser2448) antibody (Cat#R0009, 1:2,000). Green, isotype control; red, Phospho-mTOR (Ser2448).
  • Validation of mTOR knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Phospho-mTOR (Ser2448) antibody (Cat#R0009, 1:2,000) and analyzed using BD flow cytometer.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MTOR
  • Catalog：
    R0009
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using anti-phospho-mTOR (Ser2448) antibody (Cat#R0009). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-mTOR (Ser2448) antibody (Cat#R0009, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-phospho-mTOR (Ser2448) antibody (Cat#R0009). Phospho-mTOR (Ser2448) expression in wild-type (WT) and MTOR shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-mTOR (Ser2448) antibody (Cat#R0009, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with anti-Phospho-mTOR (Ser2448) antibody (Cat#R0009, 1:1,000). Nuclei were stained blue with DAPI; Phospho-mTOR (Ser2448) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using anti-Phospho-mTOR (Ser2448) antibody (Cat#R0009, 1:1,000), Top panel: wild-type (WT); Bottom panal: Phospho-mTOR (Ser2448) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-mTOR (Ser2448) was stained magenta with Alexa Fluor® 647. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-mTOR (Ser2448) expression in HepG2 cells using anti-Phospho-mTOR (Ser2448) antibody (Cat#R0009, 1:2,000). Green, isotype control; red, Phospho-mTOR (Ser2448).
Validation of mTOR knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Phospho-mTOR (Ser2448) antibody (Cat#R0009, 1:2,000) and analyzed using BD flow cytometer.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$
View cart Empty cart
  • Phone Number

    (540)855-7041

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.