KD-Validated JNK1 Rabbit mAb - Product - Hefei GenuIN Biotech Co., Ltd.

KD-Validated JNK1 Rabbit mAb

#R0012

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Knockdown Validated

Western blotting analysis using anti-JNK1 antibody (Cat#R0012). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-JNK1 antibody (Cat#R0012, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-JNK1 antibody (Cat#R0012). JNK1 expression in wild-type (WT) and MAPK8 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-JNK1 antibody (Cat#R0012, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Flow cytometric analysis of JNK1 expression in C2C12 cells using anti-JNK1 antibody (Cat#R0012, 1:2,000). Green, isotype control; red, JNK1.

Validation of JNK1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-JNK1 antibody (Cat#R0012, 1:2,000) and analyzed using BD flow cytometer.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

Mapk8
Catalog：

R0012
Application：

WB, FCM
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KD-Validated JNK1 Rabbit mAb
Aliases	MAPK8; Mitogen-Activated Protein Kinase 8; SAPK1; JNK1; PRKM8; JNK; Stress-Activated Protein Kinase 1c; C-Jun N-Terminal Kinase 1; JUN N-Terminal Kinase; MAP Kinase 8; EC 2.7.11.24; JNK-46; SAPK1c; Stress-Activated Protein Kinase JNK1; Stress-Activated Protein Kinase 1; JNK21B1/2; EC 2.7.11; JNK1A2; SAPK1C; MAPK 8
Background	Gene Name: Mapk8 NCBI Gene Entry: 5599 UniProt Entry: P45983
Application Information	Molecular Weight: Predicted, 44 kDa; observed, 42-50 kDa Clonality: Rabbit monoclonal antibody Clone ID: S-1153-21 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	N/A
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using anti-JNK1 antibody (Cat#R0012). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-JNK1 antibody (Cat#R0012, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-JNK1 antibody (Cat#R0012). JNK1 expression in wild-type (WT) and MAPK8 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-JNK1 antibody (Cat#R0012, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of JNK1 expression in C2C12 cells using anti-JNK1 antibody (Cat#R0012, 1:2,000). Green, isotype control; red, JNK1.
Validation of JNK1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-JNK1 antibody (Cat#R0012, 1:2,000) and analyzed using BD flow cytometer.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

Mapk8
Catalog：

R0012
Application：

WB, FCM
Species Reactivity：

H, M, R

Datasheet

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