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Home>Product>Primary Antibody

Phospho-Erk1/2 (T202/Y204) Rabbit mAb

#R0014

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Western blotting analysis using anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cat#R0014). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cat#R0014, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cat#R0014, 1:1,000). Nuclei were stained blue with DAPI; Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) expression in C2C12 cells using anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cat#R0014, 1:2,000). Green, isotype control; red, Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MAPK3/MAPK1
  • Catalog：
    R0014
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospho-Erk1/2 (T202/Y204) Rabbit mAb
Aliases MAPK3; Mitogen-Activated Protein Kinase 3; ERK1; PRKM3; Extracellular Signal-Regulated Kinase 1; Microtubule-Associated Protein 2 Kinase; Insulin-Stimulated MAP2 Kinase; EC 2.7.11.24; P44-ERK1; P44-MAPK; P44ERK1; P44MAPK; ERK-1; ERT2; Extracellular Signal-Related Kinase 1; MAP Kinase Isoform P44; MAP Kinase; EC 2.7.11; HS44KDAP; HUMKER1A; P44mapk; P44erk1; MAPK 1; MAPK 3; MAPK1; Mitogen-Activated Protein Kinase 1; ERK2; Extracellular Signal-Regulated Kinase 2; MAPK2; PRKM1; PRKM2; ERK; Mitogen-Activated Protein Kinase 2; MAP Kinase 1; MAP Kinase 2; EC 2.7.11.24; P42-MAPK; P41mapk; MAPK; ERK-2; ERT1; Protein Tyrosine Kinase; ERK2; MAP Kinase Isoform P42; EC 2.7.11; P42MAPK; P41MAPK; MAPK 1; NS13; P38; P40; P41
Background

Gene Name: MAPK3/MAPK1

NCBI Gene Entry: 5595/5594

UniProt Entry: P27361/P28482

Application Information

Molecular Weight: Predicted, 43,41 kDa; observed, 38,40 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: S-602-276

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Erk1/2 (T202/Y204)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cat#R0014). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cat#R0014, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of Hela cells with anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cat#R0014, 1:1,000). Nuclei were stained blue with DAPI; Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Flow cytometric analysis of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) expression in C2C12 cells using anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cat#R0014, 1:2,000). Green, isotype control; red, Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MAPK3/MAPK1
  • Catalog：
    R0014
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cat#R0014). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cat#R0014, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cat#R0014, 1:1,000). Nuclei were stained blue with DAPI; Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) expression in C2C12 cells using anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cat#R0014, 1:2,000). Green, isotype control; red, Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204).
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