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GST Tag Mouse mAb

#T023

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Western blotting analysis using His tag antibody (Cat#T023). GST-tagged fusion protein expressed by E. coli were loaded and separated by SDS-PAGE. The blot was incubated with His tag antibody (Cat#T023, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
  • Catalog:
    T023
  • Application:
    WB
  • Species Reactivity:
    Recombinant protein
Information
Product Name GST Tag Mouse mAb
Aliases Glutathione S Transferase mu; glutathione-S-transferase; Glutathione S Transferase; Glutathione S transferase Mu 1; GST 26; GST class mu 1; GST1; GSTM1; GSTM1 1; GSTM1a 1a; GSTM1b 1b; GTH4; GTM1; H B; HB subunit 4; MGC26563; MU 1; MU; SJ26 antigen; SjGST; GST-tag
Background The GST Tag (Glutathione S-Transferase tag) is a larger affinity tag (~26 kDa) derived from the Schistosoma japonicum enzyme glutathione S-transferase. It is genetically fused to the N-terminus or C-terminus of recombinant proteins, primarily serving as a purification and solubility tag. Unlike smaller epitope tags (e.g., HA or Myc), GST leverages enzymatic activity for affinity-based isolation. Its size can influence protein folding or function but often enhances solubility and stability of fused partners, especially for insoluble or unstable proteins.
Application Information

Molecular Weight: Recombinant protein dependent

Clonality: Mouse monoclonal antibody

Clone ID: 25GB7025

Species Reactivity: Recombinant protein

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of GST-Tag
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.05% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using His tag antibody (Cat#T023). GST-tagged fusion protein expressed by E. coli were loaded and separated by SDS-PAGE. The blot was incubated with His tag antibody (Cat#T023, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using His tag antibody (Cat#T023). GST-tagged fusion protein expressed by E. coli were loaded and separated by SDS-PAGE. The blot was incubated with His tag antibody (Cat#T023, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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