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Home>Product>Primary Antibody

KD-Validated IKBKG Mouse mAb

#65173

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Knockdown Validated
Western blotting analysis using IKBKG antibody (Cat#65173). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IKBKG antibody (Cat#65173, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using IKBKG antibody (Cat#65173). IKBKG expression in wild-type (WT) and IKBKG knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with IKBKG antibody (Cat#65173, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of IKBKG expression in HeLa cells using IKBKG antibody (Cat#65173, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, IKBKG.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    IKBKG
  • Catalog：
    65173
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated IKBKG Mouse mAb
Aliases IKBKG; Inhibitor Of Nuclear Factor Kappa B Kinase Regulatory Subunit Gamma; IKK-Gamma; IKKAP1; FIP-3; NEMO; FIP3; IKKG; IkB Kinase-Associated Protein 1; NF-Kappa-B Essential Modulator; I-Kappa-B Kinase Subunit Gamma; IkB Kinase Subunit Gamma; ZC2HC9; Fip3p; Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells, Kinase Gamma; Inhibitor Of Nuclear Factor Kappa B Kinase Subunit Gamma; 14.7K (Adenovirus E3 Protein) Interacting Protein 3; NF-Kappa-B Essential Modifier; Incontinentia Pigmenti; IP1; IP2; Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Gamma; AMCBX1; EDAID1; IMD33; SAIDX ; IPD2; IP
Background

Gene Name: IKBKG

NCBI Gene Entry: 8517

UniProt Entry: Q9Y6K9

Application Information

Molecular Weight: Predicted, 48 kDa; observed, 48 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB14145

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human IKBKG
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:200-1:1,000
Flow Cytometry (FCM): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using IKBKG antibody (Cat#65173). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IKBKG antibody (Cat#65173, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using IKBKG antibody (Cat#65173). IKBKG expression in wild-type (WT) and IKBKG knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with IKBKG antibody (Cat#65173, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of IKBKG expression in HeLa cells using IKBKG antibody (Cat#65173, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, IKBKG.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    IKBKG
  • Catalog：
    65173
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using IKBKG antibody (Cat#65173). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IKBKG antibody (Cat#65173, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using IKBKG antibody (Cat#65173). IKBKG expression in wild-type (WT) and IKBKG knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with IKBKG antibody (Cat#65173, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of IKBKG expression in HeLa cells using IKBKG antibody (Cat#65173, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, IKBKG.
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