Western blotting analysis using Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796). Total cell lysates (20 ug) from various breast cancer cell lines were loaded and separated by SDS-PAGE. The membrane was incubated with the respective primary antibody and corresponding HRP-conjugated secondary antibody. Images were developed using Clarity Western ECL Substrate. For reference, we use the Bio-Rad Dual Protein Ladder and the provided ladder images are merged colorimetric. Included for each image is expected observed protein sizes, the antibody dilution, and chemiluminescent exposure time.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human Phospho-ERK1 (Y204) + ERK2 (Y187)
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796). Total cell lysates (20 ug) from various breast cancer cell lines were loaded and separated by SDS-PAGE. The membrane was incubated with the respective primary antibody and corresponding HRP-conjugated secondary antibody. Images were developed using Clarity Western ECL Substrate. For reference, we use the Bio-Rad Dual Protein Ladder and the provided ladder images are merged colorimetric. Included for each image is expected observed protein sizes, the antibody dilution, and chemiluminescent exposure time.
Western blotting analysis using Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796). Total cell lysates (20 ug) from various breast cancer cell lines were loaded and separated by SDS-PAGE. The membrane was incubated with the respective primary antibody and corresponding HRP-conjugated secondary antibody. Images were developed using Clarity Western ECL Substrate. For reference, we use the Bio-Rad Dual Protein Ladder and the provided ladder images are merged colorimetric. Included for each image is expected observed protein sizes, the antibody dilution, and chemiluminescent exposure time.
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