Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using histone H3.3 antibody (Cat#2120, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Western blotting analysis using Histone H3.3 antibody (Cat#2120). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Histone H3.3 antibody (Cat#2120, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Histone H3.3 expression in HepG2 cells using Histone H3.3 antibody (Cat#2120, 1:2,000). Green, isotype control; red, Histone H3.3.
Immunocytochemical staining of HepG2 cells with Histone H3.3 antibody (Cat#2120, 1:1,000). Nuclei were stained blue with DAPI; Histone H3.3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using histone H3.3 antibody (Cat#2120, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Western blotting analysis using Histone H3.3 antibody (Cat#2120). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Histone H3.3 antibody (Cat#2120, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Histone H3.3 expression in HepG2 cells using Histone H3.3 antibody (Cat#2120, 1:2,000). Green, isotype control; red, Histone H3.3.
Immunocytochemical staining of HepG2 cells with Histone H3.3 antibody (Cat#2120, 1:1,000). Nuclei were stained blue with DAPI; Histone H3.3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using histone H3.3 antibody (Cat#2120, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Western blotting analysis using Histone H3.3 antibody (Cat#2120). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Histone H3.3 antibody (Cat#2120, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Histone H3.3 expression in HepG2 cells using Histone H3.3 antibody (Cat#2120, 1:2,000). Green, isotype control; red, Histone H3.3.
Immunocytochemical staining of HepG2 cells with Histone H3.3 antibody (Cat#2120, 1:1,000). Nuclei were stained blue with DAPI; Histone H3.3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
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