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  • Primary Antibody
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Home>Product>Primary Antibody

KD-Validated Grp75 Recombinant Rabbit mAb

#61525

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using Grp75 antibody (Cat#61525). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Grp75 antibody (Cat#61525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Grp75 antibody (Cat#61525). Grp75 expression in wild type (WT) and Grp75 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Grp75 antibody (Cat#61525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Grp75 antibody (Cat#61525, 1:1,000). Nuclei were stained blue with DAPI; Grp75 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    HSPA9
  • Catalog：
    61525
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name KD-Validated Grp75 Recombinant Rabbit mAb
Aliases HSPA9; Heat Shock Protein Family A (Hsp70) Member 9; GRP75; PBP74; 75 KDa Glucose-Regulated Protein; Stress-70 Protein, Mitochondrial; HSPA9B; Heat Shock 70kDa Protein 9 (Mortalin); Peptide-Binding Protein 74; Mortalin2; Mortalin; MTHSP75; GRP-75; MOT; Epididymis Secretory Sperm Binding Protein Li 124m; Heat Shock 70kDa Protein 9B (Mortalin-2); Catecholamine-Regulated Protein 40; Heat Shock 70 KDa Protein 9; Heat Shock 70kD Protein 9B; Mortalin, Perinuclear; P66-Mortalin; HEL-S-124m; Mortalin-2; Mt-HSP70; Mthsp75; SIDBA4; Mot-2; CRP40; EVPLS; MOT-2; MOT2; SAAN; CSA
Background

Gene Name: HSPA9

NCBI Gene Entry: 3313

UniProt Entry: P38646

Application Information

Molecular Weight: Predicted, 74 kDa, observed, 74 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2615

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Grp75
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Grp75 antibody (Cat#61525). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Grp75 antibody (Cat#61525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using Grp75 antibody (Cat#61525). Grp75 expression in wild type (WT) and Grp75 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Grp75 antibody (Cat#61525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Grp75 antibody (Cat#61525, 1:1,000). Nuclei were stained blue with DAPI; Grp75 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    HSPA9
  • Catalog：
    61525
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using Grp75 antibody (Cat#61525). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Grp75 antibody (Cat#61525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Grp75 antibody (Cat#61525). Grp75 expression in wild type (WT) and Grp75 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Grp75 antibody (Cat#61525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Grp75 antibody (Cat#61525, 1:1,000). Nuclei were stained blue with DAPI; Grp75 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

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    8:30 AM - 5:30 PM EST Mon-Fri

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Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

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  144. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/contract/TemplateHandlerInterface.php ( 1.60 KB )
  145. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public.php ( 3.56 KB )
  146. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_param.php ( 0.75 KB )
  147. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Request.php ( 9.20 KB )
  148. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/mobile.php ( 4.08 KB )
  149. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
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  158. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-trace/src/Html.php ( 4.42 KB )
  1. CONNECT:[ UseTime:0.000621s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.004931s ]
  3. SELECT * FROM `goods` WHERE `code` = '61525' LIMIT 1 [ RunTime:0.002980s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001140s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.003713s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000784s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 7420 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001411s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.006041s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 7420 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000388s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000948s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.003186s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.183734s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5497,8423,9069,6634,4636,4894,9476,8043,4670,6737) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001537s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000779s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000535s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.003291s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000208s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000332s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000352s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000214s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.002222s ]
0.329822s