Western blotting analysis using NPC1 antibody (Cat#2391). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NPC1 antibody (Cat#2391, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of NPC1 expression in HT-1080 cells using NPC1 antibody (Cat#2391, 1:2,000). Green, isotype control; red, NPC1.
Immunocytochemical staining of HT-1080 cells with NPC1 antibody (Cat#2391, 1:1,000). Nuclei were stained blue with DAPI; NPC1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using NPC1 antibody (Cat#2391). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NPC1 antibody (Cat#2391, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of NPC1 expression in HT-1080 cells using NPC1 antibody (Cat#2391, 1:2,000). Green, isotype control; red, NPC1.
Immunocytochemical staining of HT-1080 cells with NPC1 antibody (Cat#2391, 1:1,000). Nuclei were stained blue with DAPI; NPC1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using NPC1 antibody (Cat#2391). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NPC1 antibody (Cat#2391, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of NPC1 expression in HT-1080 cells using NPC1 antibody (Cat#2391, 1:2,000). Green, isotype control; red, NPC1.
Immunocytochemical staining of HT-1080 cells with NPC1 antibody (Cat#2391, 1:1,000). Nuclei were stained blue with DAPI; NPC1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
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