Information
Data
-
Hela cells were stained with anti-CTCF antibody and mounted in FadeStop™ fluorescentmounting medium. lmages were taken 1 day (D1), 2 months (M2), and 4 months(M4) after staining using Zeiss LSM 880 confocal laser scanning microscope. Nuclei werestained blue with DAPl. ACTB was stained magenta with Alexa Fluor® 647. Scale bars, 20 um.
-
Hela cells were stained with CTCF antibody and mounted in FadeStop™ fluorescent mounting medium. The slide was stored at -20°C for 1 month before imaging using a ZeissLSM 880 confocal laser scanning microscope. A series of scans were performed to assess thephotobleaching speed of the fluorescent dye. Note that even after 12th can, the image quality was still high. Scale bars, 20 μm.