Western blotting analysis using TLE1 antibody (Cat#2742). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TLE1 antibody (Cat#2742, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using TLE1 antibody (Cat#2742, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunocytochemical staining of Hela cells with TLE1 antibody (Cat#2742, 1:1,000). Nuclei were stained blue with DAPI; TLE1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of TLE1 expression in Hela cells using TLE1 antibody (Cat#2742, 1:2,000). Green, isotype control; red, TLE1.
TLE1; TLE Family Member 1, Transcriptional Corepressor; ESG1; TLE-1; GRG1; ESG; Transducin-Like Enhancer Of Split 1 (E(Sp1) Homolog, Drosophila); Enhancer Of Split Groucho-Like Protein 1; Transducin Like Enhancer Of Split 1; Transducin-Like Enhancer Protein 1; Enhancer Of Split Groucho 1; E(Sp1) Homolog
Western blotting analysis using TLE1 antibody (Cat#2742). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TLE1 antibody (Cat#2742, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using TLE1 antibody (Cat#2742, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunocytochemical staining of Hela cells with TLE1 antibody (Cat#2742, 1:1,000). Nuclei were stained blue with DAPI; TLE1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of TLE1 expression in Hela cells using TLE1 antibody (Cat#2742, 1:2,000). Green, isotype control; red, TLE1.
Western blotting analysis using TLE1 antibody (Cat#2742). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TLE1 antibody (Cat#2742, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using TLE1 antibody (Cat#2742, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunocytochemical staining of Hela cells with TLE1 antibody (Cat#2742, 1:1,000). Nuclei were stained blue with DAPI; TLE1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of TLE1 expression in Hela cells using TLE1 antibody (Cat#2742, 1:2,000). Green, isotype control; red, TLE1.
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