Western blotting analysis using Butyrylcholinesterase antibody (Cat#2984). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Butyrylcholinesterase antibody (Cat#2984, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Butyrylcholinesterase expression in HepG2 cells using Butyrylcholinesterase antibody (Cat#2984, 1:2,000). Green, isotype control; red, Butyrylcholinesterase.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human BCHE
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using Butyrylcholinesterase antibody (Cat#2984). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Butyrylcholinesterase antibody (Cat#2984, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Butyrylcholinesterase expression in HepG2 cells using Butyrylcholinesterase antibody (Cat#2984, 1:2,000). Green, isotype control; red, Butyrylcholinesterase.
Western blotting analysis using Butyrylcholinesterase antibody (Cat#2984). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Butyrylcholinesterase antibody (Cat#2984, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Butyrylcholinesterase expression in HepG2 cells using Butyrylcholinesterase antibody (Cat#2984, 1:2,000). Green, isotype control; red, Butyrylcholinesterase.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000797s ]
SELECT * FROM `goods` WHERE `code` = '2984' LIMIT 1 [ RunTime:0.000512s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000419s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000374s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000363s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 4727 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000342s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000426s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 4727 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000275s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000438s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000256s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.053490s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (6256,6895,7603,195,4187,4525,4678,92,4584,6578) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000902s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000436s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000223s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000503s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000246s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000332s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000382s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000229s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000213s ]
