Western blotting analysis using SATB2 antibody (Cat#3131). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SATB2 antibody (Cat#3131, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of SATB2 expression in HeLa cells using SATB2 antibody (Cat#3131, 1:2,000). Green, isotype control; red, SATB2.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using SATB2 antibody (Cat#3131, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
SATB2; SATB Homeobox 2; KIAA1034; Special AT-Rich Sequence-Binding Protein 2; DNA-Binding Protein SATB2; SATB Family Member 2; FLJ21474; SATB2 Fusion; C2DELq32q33; DEL2Q32Q33; GLSS
Western blotting analysis using SATB2 antibody (Cat#3131). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SATB2 antibody (Cat#3131, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of SATB2 expression in HeLa cells using SATB2 antibody (Cat#3131, 1:2,000). Green, isotype control; red, SATB2.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using SATB2 antibody (Cat#3131, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Western blotting analysis using SATB2 antibody (Cat#3131). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SATB2 antibody (Cat#3131, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of SATB2 expression in HeLa cells using SATB2 antibody (Cat#3131, 1:2,000). Green, isotype control; red, SATB2.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using SATB2 antibody (Cat#3131, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001150s ]
SELECT * FROM `goods` WHERE `code` = '3131' LIMIT 1 [ RunTime:0.002506s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.002228s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001144s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.001574s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 4870 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000885s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.002733s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 4870 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001742s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000740s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.001509s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.200332s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4021,4357,5422,7209,8554,6630,5615,5146,8064,7010) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001835s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000683s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.001836s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.001504s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.001075s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000603s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.003230s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.002354s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000901s ]
