Western blotting analysis using phospho-STAT3 (Ser727) antibody (Cat#3232). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-STAT3 (Ser727) antibody (Cat#3232, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Phospho-STAT3 (Ser727) expression in HepG2 cells using Phospho-STAT3 (Ser727) antibody (Cat#3232, 1:2,000). Green, isotype control; red,Phospho-STAT3 (Ser727).
Immunocytochemical staining of HepG2 cells with phospho-STAT3 (Ser727) antibody (Cat#3232, 1:1,000). Nuclei were stained blue with DAPI; Phospho-STAT3 (Ser727) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
STAT3; Signal Transducer And Activator Of Transcription 3; APRF; Acute-Phase Response Factor; Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor); DNA-Binding Protein APRF; ADMIO1; ADMIO; HIES
Western blotting analysis using phospho-STAT3 (Ser727) antibody (Cat#3232). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-STAT3 (Ser727) antibody (Cat#3232, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Phospho-STAT3 (Ser727) expression in HepG2 cells using Phospho-STAT3 (Ser727) antibody (Cat#3232, 1:2,000). Green, isotype control; red,Phospho-STAT3 (Ser727).
Immunocytochemical staining of HepG2 cells with phospho-STAT3 (Ser727) antibody (Cat#3232, 1:1,000). Nuclei were stained blue with DAPI; Phospho-STAT3 (Ser727) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using phospho-STAT3 (Ser727) antibody (Cat#3232). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-STAT3 (Ser727) antibody (Cat#3232, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Phospho-STAT3 (Ser727) expression in HepG2 cells using Phospho-STAT3 (Ser727) antibody (Cat#3232, 1:2,000). Green, isotype control; red,Phospho-STAT3 (Ser727).
Immunocytochemical staining of HepG2 cells with phospho-STAT3 (Ser727) antibody (Cat#3232, 1:1,000). Nuclei were stained blue with DAPI; Phospho-STAT3 (Ser727) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
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