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  • Primary Antibody
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Home>Product>Primary Antibody

Phospho-Smad1 (Ser463/Ser465) Recombinant Rabbit mAb

#3330

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Recombinant Antibody
Western blotting analysis using phospho-smad1 (Ser463/Ser465) antibody (Cat#3330). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-smad1 (Ser463/Ser465) antibody (Cat#3330, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Phospho-Smad1 (Ser463/Ser465) expression in HAP-1 cells using Phospho-Smad1 (Ser463/Ser465) antibody (Cat#3330, 1:2,000). Green, isotype control; red, Phospho-Smad1 (Ser463/Ser465).
Immunocytochemical staining of HAP-1 cells with Phospho-Smad1 (Ser463/Ser465) antibody (Cat#3330, 1:1,000). Nuclei were stained blue with DAPI;Phospho-Smad1 (Ser463/Ser465) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    SMAD1
  • Catalog：
    3330
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospho-Smad1 (Ser463/Ser465) Recombinant Rabbit mAb
Aliases SMAD1; SMAD Family Member 1; MADR1; JV4-1; MADH1; Mothers Against Decapentaplegic Homolog 1; Mothers Against DPP Homolog 1; Mad-Related Protein 1; BSP-1; BSP1; MAD, Mothers Against Decapentaplegic Homolog 1 (Drosophila); Transforming Growth Factor-Beta Signaling Protein 1; Transforming Growth Factor-Beta-Signaling Protein 1; SMAD, Mothers Against DPP Homolog 1 (Drosophila); MAD, Mothers Against Decapentaplegic Homolog 1; SMAD, Mothers Against DPP Homolog 1; TGF-Beta Signaling Protein 1; MAD Homolog 1; SMAD 1; HSMAD1; Smad1; JV41
Background

Gene Name: SMAD1

NCBI Gene Entry: 4086

UniProt Entry: Q15797

Application Information

Molecular Weight: Predicted, 52 kDa, observed, 52 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5090

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-Smad1 (Ser463/Ser465)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using phospho-smad1 (Ser463/Ser465) antibody (Cat#3330). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-smad1 (Ser463/Ser465) antibody (Cat#3330, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of Phospho-Smad1 (Ser463/Ser465) expression in HAP-1 cells using Phospho-Smad1 (Ser463/Ser465) antibody (Cat#3330, 1:2,000). Green, isotype control; red, Phospho-Smad1 (Ser463/Ser465).
  • Immunocytochemical staining of HAP-1 cells with Phospho-Smad1 (Ser463/Ser465) antibody (Cat#3330, 1:1,000). Nuclei were stained blue with DAPI;Phospho-Smad1 (Ser463/Ser465) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    SMAD1
  • Catalog：
    3330
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using phospho-smad1 (Ser463/Ser465) antibody (Cat#3330). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-smad1 (Ser463/Ser465) antibody (Cat#3330, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Phospho-Smad1 (Ser463/Ser465) expression in HAP-1 cells using Phospho-Smad1 (Ser463/Ser465) antibody (Cat#3330, 1:2,000). Green, isotype control; red, Phospho-Smad1 (Ser463/Ser465).
Immunocytochemical staining of HAP-1 cells with Phospho-Smad1 (Ser463/Ser465) antibody (Cat#3330, 1:1,000). Nuclei were stained blue with DAPI;Phospho-Smad1 (Ser463/Ser465) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  144. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/contract/TemplateHandlerInterface.php ( 1.60 KB )
  145. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public.php ( 3.56 KB )
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  147. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Request.php ( 9.20 KB )
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  149. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
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  1. CONNECT:[ UseTime:0.000522s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000714s ]
  3. SELECT * FROM `goods` WHERE `code` = '3330' LIMIT 1 [ RunTime:0.000533s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000527s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000378s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000416s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 4967 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000363s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000468s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 4967 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000337s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000370s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000263s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.054714s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (7028,7180,7632,6408,5813,154,9205,3738,7878,6204) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000934s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000545s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000339s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000538s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000345s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000311s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000393s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000282s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000242s ]
0.096201s