Western blotting analysis using GST3 / GSTP1 antibody (Cat#62189). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GST3 / GSTP1 antibody (Cat#62189, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using GST3 / GSTP1 antibody (Cat#62189). GST3 / GSTP1 expression in wild type (WT) and GST3 / GSTP1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with GST3 / GSTP1 antibody (Cat#62189, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of GST3 / GSTP1 expression in HAP-1 cells using GST3 / GSTP1 antibody (Cat #62189, 1:2,000). Green, isotype control; red, GST3 / GSTP1.
Immunocytochemical staining of HAP-1 cells with GST3 / GSTP1 antibody (Cat#62189, 1:1,000) . Nuclei were stained blue with DAPI; GST3 / GSTP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using GST3/GSTP1 antibody (Cat#62189, 1:1,000), Top panel: wild-type (WT); Bottom panal: GSTP1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; GST3/GSTP1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
