Western blotting analysis using AKT1 antibody (Cat#3447). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AKT1 antibody (Cat#3447, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Flow cytometric analysis of AKT1 expression in C2C12 cells using AKT1 antibody (Cat#3447, 1:2,000). Green, isotype control; red, AKT1.
Immunocytochemical staining of C2C12 cells with AKT1 antibody (Cat#3447, 1:1,000). Nuclei were stained blue with DAPI;AKT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen
Recombinant protein of human AKT1
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB):1:2,500-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000 Immunocytochemistry (ICC): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using AKT1 antibody (Cat#3447). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AKT1 antibody (Cat#3447, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Flow cytometric analysis of AKT1 expression in C2C12 cells using AKT1 antibody (Cat#3447, 1:2,000). Green, isotype control; red, AKT1.
Immunocytochemical staining of C2C12 cells with AKT1 antibody (Cat#3447, 1:1,000). Nuclei were stained blue with DAPI;AKT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using AKT1 antibody (Cat#3447). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AKT1 antibody (Cat#3447, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Flow cytometric analysis of AKT1 expression in C2C12 cells using AKT1 antibody (Cat#3447, 1:2,000). Green, isotype control; red, AKT1.
Immunocytochemical staining of C2C12 cells with AKT1 antibody (Cat#3447, 1:1,000). Nuclei were stained blue with DAPI;AKT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
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