Western blotting analysis using EIF3D antibody (Cat#3871). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF3D antibody (Cat#3871, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with EIF3D antibody (Cat#3871, 1:500). Nuclei were stained blue with DAPI; EIF3D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen
Recombinant protein of human EIF3D
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:2,500 Immunocytochemistry (ICC): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using EIF3D antibody (Cat#3871). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF3D antibody (Cat#3871, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with EIF3D antibody (Cat#3871, 1:500). Nuclei were stained blue with DAPI; EIF3D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using EIF3D antibody (Cat#3871). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF3D antibody (Cat#3871, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with EIF3D antibody (Cat#3871, 1:500). Nuclei were stained blue with DAPI; EIF3D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001630s ]
SELECT * FROM `goods` WHERE `code` = '3871' LIMIT 1 [ RunTime:0.001575s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000683s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001156s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000749s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5234 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001402s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000571s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5234 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000840s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000823s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000674s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.089689s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (9823,7761,9778,9373,5736,330,282,5926,5208,6630) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000743s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000413s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000206s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000459s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000288s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000456s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000520s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000329s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000301s ]
