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  • Primary Antibody
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Home>Product>Primary Antibody

Alpha Smooth Muscle Actin Recombinant Rabbit mAb

#4018

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Recombinant Antibody
Western blotting analysis using alpha smooth muscle Actin antibody (Cat#4018). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with alpha smooth muscle Actin antibody (Cat#4018, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of alpha smooth muscle Actin expression in C2C12 cells using alphasmoothmuscleActin antibody (Cat#4018, 1:2,000). Green, isotype control; red, alpha smooth muscle Actin.
Immunocytochemical staining of C2C12 cells with alpha smooth muscle Actin antibody (Cat#4018, 1:1,000). Nuclei were stained blue with DAPI; alpha smooth muscle Actin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using alpha smooth muscle actin antibody (Cat#4018, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    ACTA2
  • Catalog：
    4018
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Alpha Smooth Muscle Actin Recombinant Rabbit mAb
Aliases ACTA2; Actin Alpha 2, Smooth Muscle; ACTSA; Cell Growth-Inhibiting Gene 46 Protein; Actin, Alpha 2, Smooth Muscle, Aorta; Actin, Aortic Smooth Muscle; Alpha-Cardiac Actin; Alpha-Actin-2; EC 3.6.4.-; ACTVS; α-SMA; alpha smooth muscle Actin; a SMA
Background

Gene Name: ACTA2

NCBI Gene Entry: 59

UniProt Entry: P62736

Application Information

Molecular Weight: Predicted, 42 kDa, observed, 42 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB9180

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human alpha smooth muscle Actin
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:4,000-1:20,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using alpha smooth muscle Actin antibody (Cat#4018). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with alpha smooth muscle Actin antibody (Cat#4018, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of alpha smooth muscle Actin expression in C2C12 cells using alphasmoothmuscleActin antibody (Cat#4018, 1:2,000). Green, isotype control; red, alpha smooth muscle Actin.
  • Immunocytochemical staining of C2C12 cells with alpha smooth muscle Actin antibody (Cat#4018, 1:1,000). Nuclei were stained blue with DAPI; alpha smooth muscle Actin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using alpha smooth muscle actin antibody (Cat#4018, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    ACTA2
  • Catalog：
    4018
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using alpha smooth muscle Actin antibody (Cat#4018). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with alpha smooth muscle Actin antibody (Cat#4018, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of alpha smooth muscle Actin expression in C2C12 cells using alphasmoothmuscleActin antibody (Cat#4018, 1:2,000). Green, isotype control; red, alpha smooth muscle Actin.
Immunocytochemical staining of C2C12 cells with alpha smooth muscle Actin antibody (Cat#4018, 1:1,000). Nuclei were stained blue with DAPI; alpha smooth muscle Actin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using alpha smooth muscle actin antibody (Cat#4018, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
  • Phone Number

    (540)855-7041

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

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Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.

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  143. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/view/driver/Php.php ( 6.44 KB )
  144. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/contract/TemplateHandlerInterface.php ( 1.60 KB )
  145. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public.php ( 3.56 KB )
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  147. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Request.php ( 9.20 KB )
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  149. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
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  1. CONNECT:[ UseTime:0.000327s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000678s ]
  3. SELECT * FROM `goods` WHERE `code` = '4018' LIMIT 1 [ RunTime:0.000454s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000405s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000304s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000395s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 5364 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000331s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000457s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 5364 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000289s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000373s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000245s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.151927s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (9809,8551,8615,4967,4441,8219,6964,3990,9370,8319) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001706s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000619s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000513s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.008484s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.002337s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.004634s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000662s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.003506s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000228s ]
0.231784s