Western blotting analysis using HSPE1 antibody (Cat#4890). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPE1 antibody (Cat#4890, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of HSPE1 expression in HepG2 cells using HSPE1 antibody (Cat#4890, 1:1,000). Green, isotype control; red, HSPE1.
HSPE1; Heat Shock Protein Family E (Hsp10) Member 1; CPN10; EPF; Chaperonin 10; GroES; HSP10; Heat Shock 10kD Protein 1 (Chaperonin 10); 10 KDa Heat Shock Protein, Mitochondrial; Heat Shock Protein Family E Member 1; Heat Shock 10kDa Protein 1; Early-Pregnancy Factor; 10 KDa Chaperonin; Epididymis Secretory Sperm Binding Protein; Early Pregnancy Factor; Hsp10
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human Cpn10
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using HSPE1 antibody (Cat#4890). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPE1 antibody (Cat#4890, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of HSPE1 expression in HepG2 cells using HSPE1 antibody (Cat#4890, 1:1,000). Green, isotype control; red, HSPE1.
Western blotting analysis using HSPE1 antibody (Cat#4890). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPE1 antibody (Cat#4890, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of HSPE1 expression in HepG2 cells using HSPE1 antibody (Cat#4890, 1:1,000). Green, isotype control; red, HSPE1.
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