Western blotting analysis using TRIM65 antibody (Cat#5097). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TRIM65 antibody (Cat#5097, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of TRIM65 expression in HeLa cells using TRIM65 antibody (Cat#5097, 1:1,000). Green, isotype control; red, TRIM65.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human TRIM65
Isotype
Mouse IgG2b
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000 Flow Cytometry (FCM): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using TRIM65 antibody (Cat#5097). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TRIM65 antibody (Cat#5097, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of TRIM65 expression in HeLa cells using TRIM65 antibody (Cat#5097, 1:1,000). Green, isotype control; red, TRIM65.
Western blotting analysis using TRIM65 antibody (Cat#5097). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TRIM65 antibody (Cat#5097, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of TRIM65 expression in HeLa cells using TRIM65 antibody (Cat#5097, 1:1,000). Green, isotype control; red, TRIM65.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000784s ]
SELECT * FROM `goods` WHERE `code` = '5097' LIMIT 1 [ RunTime:0.000526s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000427s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000368s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000410s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6258 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000364s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000430s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6258 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000275s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000379s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000260s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.063003s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (10480,5100,5262,6436,10561,5471,59,5762,6136,5214) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000736s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000479s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000217s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000441s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000200s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000318s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000371s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000263s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000200s ]
