Western blotting analysis using VPS4A antibody (Cat#5629). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with VPS4A antibody (Cat#5629, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of VPS4A expression in HepG2 cells using VPS4A antibody (Cat#5629, 1:2,000). Green, isotype control; red, VPS4A.
VPS4A; Vacuolar Protein Sorting 4 Homolog A; VPS4-1; VPS4; SKD1A; SKD2; SKD1; Vacuolar Protein Sorting-Associated Protein 4A; FLJ22197; HVPS4; Vacuolar Protein Sorting 4 Homolog A (S. Cerevisiae); Vacuolar Protein Sorting 4A (Yeast Homolog); Vacuolar Protein Sorting 4A (Yeast); Vacuolar Protein Sorting Factor 4A; Vacuolar Sorting Protein 4; SKD1-Homolog; Protein SKD2; EC 3.6.4.6; CIMDAG
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human VPS4A
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using VPS4A antibody (Cat#5629). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with VPS4A antibody (Cat#5629, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of VPS4A expression in HepG2 cells using VPS4A antibody (Cat#5629, 1:2,000). Green, isotype control; red, VPS4A.
Western blotting analysis using VPS4A antibody (Cat#5629). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with VPS4A antibody (Cat#5629, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of VPS4A expression in HepG2 cells using VPS4A antibody (Cat#5629, 1:2,000). Green, isotype control; red, VPS4A.
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