Western blotting analysis using FOXO1 antibody (Cat#5851). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FOXO1 antibody (Cat#5851, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of FOXO1 expression in HepG2 cells using FOXO1 antibody (Cat#5851, 1:2,000). Green, isotype control; red, FOXO1.
Immunocytochemical staining of HepG2 cells with forkhead box O1 antibody (Cat#5851, 1:1,000). Nuclei were stained blue with DAPI; Forkhead box O1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using FOXO1 antibody (Cat#5851). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FOXO1 antibody (Cat#5851, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of FOXO1 expression in HepG2 cells using FOXO1 antibody (Cat#5851, 1:2,000). Green, isotype control; red, FOXO1.
Immunocytochemical staining of HepG2 cells with forkhead box O1 antibody (Cat#5851, 1:1,000). Nuclei were stained blue with DAPI; Forkhead box O1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using FOXO1 antibody (Cat#5851). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FOXO1 antibody (Cat#5851, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of FOXO1 expression in HepG2 cells using FOXO1 antibody (Cat#5851, 1:2,000). Green, isotype control; red, FOXO1.
Immunocytochemical staining of HepG2 cells with forkhead box O1 antibody (Cat#5851, 1:1,000). Nuclei were stained blue with DAPI; Forkhead box O1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
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