Western blotting analysis using FKBP8 antibody (Cat#61412). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FKBP8 antibody (Cat#61412, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using FKBP8 antibody (Cat#61412). FKBP8 expression in wild-type (WT) and FKBP8 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FKBP8 antibody (Cat#61412, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of FKBP8 expression in HepG2 cells using FKBP8 antibody (Cat#61412, 1:2,000). Green, isotype control; red, FKBP8.
Validation of FKBP8 knockdown using flow cytometry. Wild-type(WT, Blue) and FKBP8 knockdown(KD, Green) HeLa cells were stained with FKBP8 antibody(Cat#61412, 1:2,000) and analyzed using BD flow cytometer.