Western blotting analysis using Phospho-RSK1(S380) antibody (Cat#61513). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-RSK1(S380) antibody (Cat#61513, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Phospho-RSK1(S380) antibody (Cat#61513). Phospho-RSK1(S380) expression in wild type (WT) and phospho-RSK1(S380) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Phospho-RSK1(S380) antibody (Cat#61513, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using phospho-RSK1(S380) antibody (Cat#61513, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HeLa cells with Phospho-RSK1(S380) antibody (Cat#61513, 1:1,000). Nuclei were stained blue with DAPI; Phospho-RSK1(S380) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of Phospho-RSK1(S380) expression in C2C12 cells using Phospho-RSK1(S380) antibody (Cat#61513, 1:2,000). Green, isotype control; red, Phospho-RSK1(S380).
