Western blotting analysis using SUMO specific peptidase 1 antibody (Cat#61754). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SUMO specific peptidase 1 antibody (Cat#61754, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using SUMO specific peptidase 1 antibody (Cat#61754). SUMO specific peptidase 1 expression in wild-type (WT) and SUMO specific peptidase 1 (SENP1) knockdown (KD) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with SUMO specific peptidase 1 antibody (Cat#61754, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of SUMO specific peptidase 1 expression in HepG2 cells using SUMO specific peptidase 1 antibody (Cat#61754, 1:2,000). Green, isotype control; red, SUMO specific peptidase 1.
Immunocytochemical staining of HepG2 cells with SUMO specific peptidase 1 antibody (Cat#61754, 1:1,000). Nuclei were stained blue with DAPI; SUMO specific peptidase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
